-
Product Name
IRSp53 antibody
- Documents
-
Description
IRSp53 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human brain tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, human brain tissue, rat brain tissue. Observed molecular weight by Western-blot: 53 kDa
-
Tested applications
ELISA, IHC, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
BAI assOCiated protein 2 antibody; BAI1 assOCiated protein 2 antibody; BAIAP2 antibody; BAP2 antibody; Fas ligand assOCiated factor 3 antibody; FLAF3 antibody; Insulin receptor substrate p53 antibody; IRS 58 antibody; IRSp53 antibody; IRSp53/58 antibody; Protein BAP2 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of IRSp53 recombinant protein (Accession Number: NM_001385140). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111934(BAIAP2 Antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111934(BAIAP2 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-BAIAP2 (IP:Catalog No:111934, 4ug; Detection:Catalog No:111934 1:500) with mouse brain tissue lysate 2640ug.
-
Background
IRSp53 (also known as BAIAP2) is a multi-domain adaptor protein that originally identified as a 58/53-kDa substrate of insulin-receptor kinase in the hamster (PMID: 10332026). As a BAI1-binding protein, IRSp53 plays an important role in linking between membrane and cytoskeleton in the process of neuronal growth (PMID: 10343108). IRSp53 is necessary for Cdc42-mediated reorganization of the actin cytoskeleton and for Rac1-mediated membrane ruffling. Alternatively spliced isoforms of IRSp53 exist. IRSp53 has been implicated in several psychiatric disorders, including autism spectrum disorders (ASDs), schizophrenia, and attention deficit/hyperactivity disorder (ADHD) (PMID: 26275848).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"