IRBP (651-670) (human) peptide

IRBP (651-670) (human) peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-LAQGAYRTAVDLESLASQLT-OH

H-Leu-Ala-Gln-Gly-Ala-Tyr-Arg-Thr-Ala-Val-Asp-Leu-Glu-Ser-Leu-Ala-Ser-Gln-Leu-Thr-OH

20

95.7%

C₉₁H₁₅₁N₂₅O₃₂

2107.3

Synthetic

Powder

IRBP (651-670) (human), sometimes referred to as hIRBP651-670 or human IRBP651-670, is amino acid residues 651 to 670 of human interphotoreceptor retinoid binding protein (IRBP), which has been identified as a new uveitogenic epitope. IRBP (651-670) (human) is uveitogenic for mice of the H-2b haplotype and elicits experimental autoimmune uveitis (EAU) with a higher severity and incidence in C57BL/6 mice than the previously characterized IRBP (1-20) (human) epitope. Co-immunization with IRBP (1-20) (human) and IRBP (651-670) human is additive but not synergistic. IRBP (651-670) (human) appears to be an epitope naturally processed from a conserved region of native IRBP.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Mattapallil MJ, Silver PB, Cortes LM, St Leger AJ, Jittayasothorn Y, Kielczewski JL, et al. Characterization of a New Epitope of IRBP That Induces Moderate to Severe Uveoretinitis in Mice With H-2b Haplotype. Invest Ophthalmol Vis Sci. 2015;56(9):5439-49

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.