Human IL-6R/CD126 (His Tag) recombinant protein

Human IL-6R/CD126 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.

Interleukin 6 receptor

IL-6R; IL6R; MGC104991; IL-6R-1; IL6RA; CD126; IL-6R-ALPHA

P08887

IL6R; hCG_1810911

Human Cells

A DNA sequence encoding the extracellular domain of human IL6R (NP_000556.1) corresponding to amino acid (Met 1-Pro 365) was expressed with a C-terminal polyhistidine tag.

Human

The soluble form of recombinant human IL6R consists of 357 amino acids and has a predicted molecular mass of 40 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 60-65 kDa due to glycosylation.

> 90 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 μg/mL (100μl/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/ml.
2. Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED₅₀ for this effect is typically 20-80 ng/ml.