Human IL-17F (His Tag) recombinant protein

Human IL-17F (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-17F (IL-17F) is a cytokine that shares sequence similarity with IL17. The most notable role of IL-17 is it involvement in inducing and mediating proinflammatory responses. IL-17 is commonly associated with allergic responses. IL-17F is expressed by activated T cells, and was expressed only in activated CD4+ T cells and activated monocytes. IL-17F has been shown to stimulate the production of several other cytokines, including IL6 and IL8. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Recombinant human IL-17F did not stimulate the proliferation of hematopoietic progenitors or the migration of mature leukocytes. However, it markedly inhibited the angiogenesis of human endothelial cells and induced endothelial cells to produce IL-2, TGF-{beta}, and monocyte chemoattractant protein-1. IL-17F stimulates the production of other cytokines and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. It also function in inhibiting angiogenesis By similarity. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Interleukin-17F

IL17F; ML1; C87042; ML-1; CANDF6; CYTOKINE ML-1; IL-24; INTERLEUKIN 17F; INTERLEUKIN-24; MUTANT IL-17F; IL-17F

Q96PD4

IL17F

Human Cells

A DNA sequence encoding the human IL17F (NP_443104.1) (Arg 31-Gln 163) was expressed, with a polyhistidine tag at the N-terminus.

Human

The recombinant human IL17F consists of 153 amino acids and has a calculated molecular mass of 17.3 kDa. rhIL17F migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions and a 44 kDa band in non-reduced SDS-PAGE as the homodimer.

> 80 % as determined by SDS-PAGE

1. Measured by its ability to bind human IL17Ra in a functional ELISA.
2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The ED₅₀ for this effect is typically 0.5-3 μg/mL.