Imcroporin peptide

Imcroporin peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

FFSLLPSLIGGLVSAIK

Phe-Phe-Ser-Leu-Leu-Pro-Ser-Leu-Ile-Gly-Gly-Leu-Val-Ser-Ala-Ile-Lys

17

96.6%

Synthetic

Powder

The synthetic form of the peptide is C-terminally amidated (Personal communication). Likely to form a helical structure based on helical wheel projection. The peptide showed good activity against G+ bacteria such as M. Luteus AB93113, B. thuringiensis AB93066, S. aureus AB94004, B. subtilis, including MRSA with MIC in the ragne of 20-50 ug/ml). Antimicrobial activity of this peptide was also demonstrated in vivo using an animal model:mouse infected with S. aureus. Updated 5/4/2012GW. Imcroporin has Antibacterial activity. Imcroporin was found in Isometrus maculates.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Zhao Z, Ma Y, Dai C, et al. Imcroporin, a new cationic antimicrobial peptide from the venom of the scorpion Isometrus maculates. Antimicrob Agents Chemother. 2009;53(8):3472-7.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.