Cynomolgus IL13 / ALRH (His Tag) recombinant protein

Cynomolgus IL13 / ALRH (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL-13 Rα1 and IL-13 Rα2). As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses and diseases. In addition, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody is more importantly implicated as a central mediator of immunoregulatory processes in various cell types.

IL13

MGC116786; IL-13; MGC116788; P600; MGC116789; BHR1; ALRH

Q0PW92

IL13

Human Cells

A DNA sequence encoding the mature form of Cynomolgus (Macaca fascicularis) IL-13 (ABG75889.1) (Ser 21-Asn 132) was fused with a polyhistidine tag at the N-terminus.

Cynomolgus

The recombinant cynomolgus IL13 consists of 128 amino acids and has a calculated molecular mass of 14.5 kDa. The apparent molecular mass of the protein is approximately 25-35 kDa in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized cynomolgus IL-13 at 4 μg/ml (100 μl/well) can bind human IL13RA1 with a linear ranger of 0.156-2.5 μg/ml.
2. Measured in a cell proliferation assay using TF1 human erythroleukemic cells (Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.). The ED₅₀ for this effect is typically 50-200 ng/mL.