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Product Name
IGF2BP1 antibody
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Description
IGF2BP1 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human kidney tissue. Positive IF detected in A375 cells. Positive IP detected in HEK-293 cells. Positive WB detected in Jurkat cells, A375 cells, HEK-293 cells, HepG2 cells, HuH-7 cells, mouse kidney tissue, rat kidney tissue. Observed molecular weight by Western-blot: 60-70 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CRD BP antibody; CRDBP antibody; IGF II mRNA binding protein 1 antibody; IGF2 mRNA binding protein 1 antibody; IGF2BP1 antibody; IMP 1 antibody; IMP1 antibody; VICKZ family member 1 antibody; VICKZ1 antibody; ZBP 1 antibody; ZBP1 antibody; Zip code binding protein 1 antibody; Zipcode binding protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IGF2BP1 recombinant protein (Accession Number: NM_006546). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111679(IGF2BP1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis slide using Catalog No:111679(IGF2BP1 Antibody) at dilution of 1:50
IP Result of anti-IGF2BP1 (IP:Catalog No:111679, 4ug; Detection:Catalog No:111679 1:1000) with HEK-293 cells lysate 1480ug.
Immunofluorescent analysis of A375 cells using Catalog No:111679(IGF2BP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
IGF2BP1 represents insulin-like growth factor 2 mRNA binding protein 1
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References
- Wang RJ, Li JW, Bao BH. MicroRNA-873 (miRNA-873) inhibits glioblastoma tumorigenesis and metastasis by suppressing the expression of IGF2BP1. The Journal of biological chemistry. 290(14):8938-48. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"