Mouse IgG1 Fc (102 Cys/Ser) recombinant protein

Mouse IgG1 Fc (102 Cys/Ser) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

Ig gamma-1 chain C region secreted form

IGG1 FC

P01868

Ighg1; Igh-4

Human Cells

The Fc region of mouse IgG1 (P01868-1) (Val 98-Lys 324) (one aa mutation, 102 Cys / Ser) was expressed and purified.

Mouse

The recombinant mouse IgG1 Fc consists of 227 amino acids and has a predicted molecular mass of 25.8 kDa. As a result of glycosylation, the apparent molecular mass of rmFc is approximately 32 kDa in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE