Hymenochirin-1Pa peptide

Hymenochirin-1Pa peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

LKLSPKTKDTLKKVLKGAIKGAIAIASMA

Leu-Lys-Leu-Ser-Pro-Lys-Thr-Lys-Asp-Thr-Leu-Lys-Lys-Val-Leu-Lys-Gly-Ala-Ile-Lys-Gly-Ala-Ile-Ala-Ile-Ala-Ser-Met-Ala

29

97.1%

Synthetic

Powder

Active against a broad spectrum of pathogens, including multiple S. aureus, S. epidermidis, A. baumannii strains, E. coli ATCC 25726, S. maltophilia (MIC 0.6-5 uM), K. pneumoniae ATCC 700603 (MIC 10 uM), E. faecalis ATCC 29212, P. aeruginosa ATCC 27853 (MIC 20 uM), and weakly against C. albicans (MIC 80 uM). Also active against human non-small lung adenocarcinoma A549 cells, breast adenocarcinoma MDA-MB-231 cells, and colorectal adenocarcinoma HT-29 cells. Its applications as antibacterial and anticancer agents may be limited due to hemolytic ability. However, the D9K mutant is not hemolytic. In TFE, residues 6-17 and 21-28 are helical (Serra et al. 2014). Reg. 09/23/2014. The source of Hymenochirin-1Pa is skin secretions, Merlin's clawed frog, Pseudhymenochirus merlini, Africa.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Conlon JM, Prajeep M, Mechkarska M, et al. Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: insights into phylogenetic relationships among the Pipidae. Comp Biochem Physiol Part D Genomics Proteomics. 2013;8(4):352-7.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.