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Product Name
Human HAO1 (His Tag) recombinant protein
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Description
Hydroxyacid oxidase 1, also known as Glycolate oxidase, HAO1 and GOX1, is a member of the FMN-dependent alpha-hydroxy acid dehydrogenase family. HAO1 / GOX1 has 2-hydroxyacid oxidase activity. It is most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. HAO1 / GOX1 is a liver-specific peroxisomal enzyme that oxidizes glycolate to glyoxylate with concomitant production of H2O2. In Hao1 messenger RNA (mRNA), an iron-responsive element (IRE) homologous to the sequence recognized by iron regulatory proteins (IRP), key regulators of iron homeostasis, is present. Mammalian HAO1 / GOX1 is a peroxisomal protein and that the C-terminal sequence SKI acts as the targeting signal. Down-regulation of HAO1 / GOX1 expression during oxidative stress may provide a mechanism to prevent excessive H2O2 formation in liver peroxisomes and may represent the prototype of a poorly recognized but potentially relevant response to oxidative injury involving down-regulation of ROS-producing enzymes.
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Protein name
Hydroxyacid oxidase (Glycolate oxidase) 1, isoform CRA_a
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Protein short names
RP23-376O20.1; GOX; HAO-1; GOX1; MGC141211; HAO1; MGC142227; MGC142225; HAOX1
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Uniprot ID
Q9UJM8
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Gene Name
HAO1; hCG_39600
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the native human HAO1 (NP_060015.1) (Leu 2-Ile 370) was expressed, with a polyhistide tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human HAO1 consisting of 376 amino acids and migrates as an 42 kDa band in SDS-PAGE under reducing conditions as predicted.
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Purity
> 97 % as determined by SDS-PAGE
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Validations
Human HAO1 / GOX1 Protein (His Tag) SDS-PAGE
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