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Product Name
HYDIN antibody
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Description
HYDIN Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human cerebellum tissue. Positive WB detected in Transfected HEK-293 cells.
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
DKFZp434D0513 antibody; DKFZp434L0850 antibody; FLJ12871 antibody; FLJ14665 antibody; HYDIN antibody; HYDIN1 antibody; HYDIN2 antibody; KIAA1864 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HYDIN recombinant protein (Accession Number: BC028351). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111579(HYDIN Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using Catalog No:111579(HYDIN Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:111579(HYDIN Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:111579(HYDIN Antibody) at dilution of 1:50 (under 40x lens)
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Background
HYDIN (hydrocephalus inducing homolog), also named as HYDIN1 or HYDIN2, is a 5121 amino acid protein, which localizes in the cell projection HYDIN exists as seven alternatively spliced isoforms and is expressed in the ciliated ependymal cell layer of heart ventricle. HYDIN is also found in ciliated epithelial cells of developing spermatocytes, oviduct and bronchi and is required for ciliary motility. HYDIN gene mutation is involved in Ciliary dyskinesia primary 5.
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