Human VRK1 (His & GST Tag) recombinant protein

Human VRK1 (His & GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. As a serine/threonine kinase, VRK1 phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.

PCH1; PCH1A; 51PK; VACCINIA VIRUS B1R-RELATED KINASE 1; SERINE/THREONINE-PROTEIN KINASE 51PK; SERINE/THREONINE-PROTEIN KINASE VRK1; VACCINIA-RELATED KINASE 1

Q99986

Baculovirus-Insect Cells

A DNA sequence encoding the human VRK1 (Q99986) (Met 1-Lys 396) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human VRK1/GST chimera consists of 633 amino acids and has a calculated molecular mass of 73 KDa. It migrates as an approximately 65-70 KDa band in SDS-PAGE under reducing conditions.

> 88 % as determined by SDS-PAGE

No Kinase Activity