Human UBE2W (His Tag) recombinant protein

Human UBE2W (His Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

Ubiquitin-conjugating enzymes, also known as UBE2W, E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step of protein ubiquitination. The modification of protein with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2W is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It also catalyzes monoubiquitination and "Lys-11"-linked polyubiquitination. UBE2W is also considered to regulate FANCD2 monoubiquitination. UBE2W exhibits ubiquitin conjugating enzyme activity and catalyzes the monoubiquitination of PHD domain of Fanconi anemia complementation group L (FANCL). Over-expression of UBE2W in cells promotes the monoubiquitination of FANCD2 and down-regulated UBE2W markedly reduces the UV irradiation-induced but not MMC-induced FANCD2 monoubiquitination.

UBIQUITIN-CONJUGATING ENZYME E2 W; UBC-16; UBIQUITIN-PROTEIN LIGASE W; HUBC-16; PROBABLE UBIQUITIN-CONJUGATING ENZYME E2 W; UBIQUITIN CARRIER PROTEIN W; UBIQUITIN-CONJUGATING ENZYME 16; 6130401J04RIK; UBC16

E. coli

A DNA sequence encoding the human UBE2W (Q96B02-12) (Met 1-Cys 151) was expressed, with a polyhistidine tag at the N-terminus.

Human

The recombinant human UBE2W comprises 166 amino acids and has a predicted molecular mass of 19.2 kDa.. It migrates as an approximately 18KDa band in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE