Human TROP-2/TACSTD2 (His & Fc Tag) recombinant protein

Human TROP-2/TACSTD2 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.

Tumor-associated calcium signal transducer 2

TROP2; MGC141612; C80403; EGP-1; M1S1; GA7331; LY97; GP50; MGC141613; EGP1; TACSTD2; GA733-1

P09758

TACSTD2; GA733-1; M1S1; TROP2

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Thr 274) of human TROP2 (NP_002344.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human TROP2/Fc is a disulfide-linked homodimer. The reduced monomer consists of 495 amino acids and has a predicted molecular mass of 55.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTROP2/Fc monomer is approximately 65-75 kDa due to glycosylation.

> 98 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to TROP2 coated plates (10 μg/ml, 100 μl/well), approximately 90%-100% will adhere specifically.