Human TrkC/NTRK3 (His & Fc Tag) recombinant protein

Human TrkC/NTRK3 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

NT-3 growth factor receptor also known as neurotrophic tyrosine kinase receptor type 3 or TrkC tyrosine kinase or Trk-C receptor, is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC/NTRK3 is widely expressed in the developing and adult nervous system. In later embryonic development, TrkC/NTRK3 is expressed in various structures of the CNS including the caudatoputamen, septal nuclei, cerebellum, and brainstem. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. In the PNS, trkC hybridization appears to correlate, both temporally and spatially, with the outgrowth of axons toward their peripheral targets. TrkC/NTRK3 is widely expressed in the three identified branches of the mammalian nervous system and appears to correlate with the expression of NT-3, its cognate ligand. The apparent colocalization of trkC transcripts with NT-3 raises the possibility this neurotrophin exerts its trophic effects by a paracrine and/or autocrine mechanism. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in TrkC encoding gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.

Neurotrophic tyrosine kinase, receptor, type 3

NTRK3_TV3; TRKC; NTRK3; AW125844; GP145(TRKC)

Q96CY4

NTRK3; hCG_26627

Human Cells

A DNA sequence encoding the extracellular domain (Met1-Asp428) of human TrkC (NP_001007157.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human TrkC/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. It consists of 644 amino acids and has a calculated molecular mass of 72.5 kDa. As a result of glycosylation, rh TrkC/Fc monomer migrates as an approximately 110-120 kDa protein in SDS-PAGE under reducing conditions.

> 98 % as determined by SDS-PAGE

Immobilized human TrKc-Fch at 10 μg/ml (100 μl/well) can bind biotinylated human NT3 (Cat:10286-HNA), The EC₅₀ of biotinylated human NT3 (Cat:10286-HNA) is 23.4-54.6 ng/ml.