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Human TRAIL R1/CD261/TNFRSF10A (Fc Tag) recombinant protein

Cat.#: 504581

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Product Information

  • Product Name
    Human TRAIL R1/CD261/TNFRSF10A (Fc Tag) recombinant protein
  • Documents
  • Description
    Tumor necrosis factor receptor superfamily, member 10a (TRAIL R1), also known as TRAIL receptor 1 (TRAIL R1) or CD261 antigen, is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R1/CD261/TNFRSF10A serves as a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAIL R1 can promote the activation of NF-kappa-B. TRAIL R1/CD261/TNFRSF10A induces apoptosis of many transformed cell lines but not of normal tissues, even though its death domain-containing receptor, DR4, is expressed on both cell types.
  • Protein short names
    TNFRSF10A; DR4; APO2; MGC9365; TRAILR-1; TRAILR1; CD261; TRAIL R1
  • Uniprot ID
    O00220
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human TNFRSF10A (NP_003835.2) extracellular domain (Met 1-Asn 239) was fused with the Fc region of human IgG1 at the C-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human TNFRSF10A/Fc is a disulfide-linked homodimer. The reduced monomer consists of 372 amino acids and has a predicted molecular mass of 41.3 kDa. As a result of glycosylation, rh TNFRSF10A/Fc monomer migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
  • Purity
    > 92 % as determined by SDS-PAGE
  • Activity
    Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL.
    The ED50 for this effect is typically 2-10 ng/ml in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10.
  • Validations

    Human TRAIL R1 / CD261 / TNFRSF10A Protein (Fc Tag) SDS-PAGE

    Human TRAIL R1 / CD261 / TNFRSF10A Protein (Fc Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"