Human TCN2 (His Tag) recombinant protein

Human TCN2 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.

Transcobalamin-2

II; TCN2; TRANSCOBALAMIN II; TRANSCOBALAMIN 2; D22S750; RP23-453E10.1; TC; TC-2; TCN-2; TC2; D22S676; TCII; AW208754

P20062

TCN2; TC2

Human Cells

A DNA sequence encoding the human TCN2 (NP_000346.2) (Met 1-Trp 427) with a C-terminal polyhistidine tag was expressed.

Human

The secreted recombinant human TCN2 comprises 418 amino acids with a predicted molecular mass of 46.7 kDa. It migrates with the molecular weight of approximately 43 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human TCN2-His at 10μg/mL (100μL/well) can bind biotinylated mouse CD320-His (Cat:503815).
The EC₅₀ of biotinylated mouse CD320-His(Cat:503815) is 18-42 ng/mL.