Human SUPT5H Recombinant protein (GST tag)

Human SUPT5H Recombinant protein (GST tag)

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Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. {PubMed:10075709, PubMed:10199401, PubMed:10393184, PubMed:10421630, PubMed:10454543, PubMed:10757782, PubMed:10912001, PubMed:11112772, PubMed:11553615, PubMed:11809800, PubMed:12653964, PubMed:12718890, PubMed:14701750, PubMed:15136722, PubMed:15380072, PubMed:16214896, PubMed:9450929, PubMed:9514752, PubMed:9857195}.

Transcription elongation factor SPT5

SPT5; SPT5H

O00267

SUPT5H; SPT5; SPT5H

E. coli

A DNA sequence encoding the human SUPT5H (NP_003160.2) 807-1087 aa was fused with the N-terminal GST tag

Human

Not tested.