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Product Name
Human SULT1A3 (His Tag) recombinant protein
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Description
SULT1A3 belongs to the sulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are different in their tissue distributions and substrate specificities while their gene structure (number and length of exons) is similar. SULT1A3 gene encodes a phenol sulfotransferase with thermolabile enzyme activity. Four sulfotransferase genes are located on the p arm of chromosome 16; this gene and SULT1A4 arose from a segmental duplication. It is the most centromeric of the four sulfotransferase genes. Exons of this gene overlap with exons of a gene that encodes a protein containing GIY-YIG domains (GIYD1). SULT1A3 is expressed in liver, colon, kidney, lung, brain, spleen, small intestine, placenta and leukocyte. SULT1A3 is a sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.
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Protein short names
THERMOLABILE (MONOAMINE; MONOAMINE-SULFATING PHENOSULFOTRANSFERASE; DOPAMINE-SPECIFIC SULFOTRANSFERASE; HAST3; MGC117469; HAST; ST1A5; STM; PLACENTAL ESTROGEN SULFOTRANSFERASE; ARYL SULFOTRANSFERASE 1A3/1A4; ST1A3/ST1A4; M FORM) PHENOL SULFOTRANSFERASE; SULFOTRANSFERASE 1A3/1A4; M-PST; CATECHOLAMINE-SULFATING PHENOL SULFOTRANSFERASE; TL-PST; PHENOL SULFOTRANSFERASE 1A5; SULFOKINASE
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the mature form of human SULT1A3 (P50224-1) (Glu2-Leu295) was expressed with a polyhistide tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human SULT1A3 consists of 301 amino acids and predicts a molecular mass of 30.5 KDa. It migrates as an approximately 34 KDa band in SDS-PAGE under reducing conditions.
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Purity
> 94 % as determined by SDS-PAGE
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Activity
Measured by its ability to transfer sulfate from PAPS to 1-Napthol.
The specific activity is > 150 pmoles/min/μg. -
Validations
Human SULT1A3 Protein (His Tag) SDS-PAGE
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