Human SFRP4 (His Tag) recombinant protein

Human SFRP4 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. SFRP4 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain . Mouse SFRP4 is highly expressed in the ovary, and is localized to granulosa cells of periovulatory follicles and corpora lutea. It plays a critical role in placental development and implantation, and is also an important factor in the development of the decidual fibrinoid zone, and in trophoblast apoptosis.

Secreted frizzled-related protein 4

FRPHE; FRP-4; SFRP4; MGC26498; SFRP-4

Q6FHJ7

SFRP4; FRPHE

Human Cells

A DNA sequence encoding the human sFRP4 (NP_003005.2) (Met 1-Val 346) was expressed with a C-terminal polyhistidine tag.

Human

The recombinant human sFRP4 consists of 336 amino acids after removal of the signal peptide and has a predicted molecular mass of 39 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 55-60 kDa due to glycosylation.

> 90 % as determined by SDS-PAGE

Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells.
The ED₅₀ for this effect is typically 1-6 μg/mL in the presence of 20 ng/mL of Wnt3a.