-
Product Name
Human NPC1/Niemann-Pick C1 protein (His & FLAG Tag)
- Documents
-
Description
Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. {PubMed:18772377, PubMed:19563754}.; (Microbial infection) Acts as an endosomal entry receptor for ebolavirus. {PubMed:21866103, PubMed:25855742}.
-
Protein name
Niemann-Pick C1 protein
-
Uniprot ID
O15118
-
Gene Name
NPC1
-
Source/Expression Host
Human Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the human NPC1 (NP_000262.2) (Arg372-Phe622) was expressed with a N-terminal polyhistide-tagged FLAG tag at the N-terminus (his-FLAG).
-
Protein Species
Human
-
Molecular weight
The recombinant human NPC1 consists 278 amino acids and predicts a molecular mass of 32 kDa.
-
Purity
> 95 % as determined by SDS-PAGE
-
Validations
Human NPC1 Protein (His & FLAG Tag)
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"