Human NKG2D / CD314 (aa 78-216, His Tag) recombinant protein

Human NKG2D / CD314 (aa 78-216, His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

NKG2D, also known as CD314, is an immune receptor which consists of two disulphide-linked type II transmembrane proteins with short intracellular proteins uncapable to transduce signals. In order to transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.

NKG2-D; NKG2-D-ACTIVATING NK RECEPTOR; D6H12S2489E; NKG2D; CD314; D12S2489E; NK CELL RECEPTOR D; KLR; KLRK1; NKG2-D TYPE II INTEGRAL MEMBRANE PROTEIN; NATURAL KILLER CELL GROUP 2D

Baculovirus-Insect Cells

A DNA sequence encoding the human NKG2D (Phe78-Val216) was fused with a polyhistide tag at the N-terminus.

Human

The recombinant human NKG2D consists of 157 amino acids and has a calculated molecular mass of 18.4 kDa.

> 90 % as determined by SDS-PAGE

1. Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-Fch (Cat:502650), The EC₅₀ of human ULBP1-Fch (Cat:502650) is 0.04-0.08 μg/ml.
2. Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch (Cat:503201), The EC₅₀ of human MICB-Fch (Cat:503201) is 15.9-37.1 ng/ml.