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Product Name
Human ITGA8 & ITGB1 recombinant protein
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Uniprot ID
P53708
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Leu 1012) of human ITGA8 (P53708) was fused with a flag tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the extracellular domain (Met 1-Asp 728) of human ITGB1 (P05556-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified
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Protein Species
Human
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Molecular weight
The recombinant heterodimer of human ITGA8/ITGB1 comprises 1765 (1016 + 749) amino acids and has a calculated molecular mass of 196 (113 + 83) kDa. As a result of glycosylation, the apparent molecular mass of rh ITGA8/ITGB1 heterodimer is approximately 120 &140 kDa in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Validations
Human ITGA8 & ITGB1 Heterodimer Protein SDS-PAGE
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