Human IL7 / interleukin-7 recombinant protein

Human IL7 / interleukin-7 recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

IL7, also known as interleukin 7, is a hematopoietic growth factor which belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes.

A630026I06RIK; IL-7; INTERLEUKIN 7; HLB368; IL7

E. coli

A DNA sequence encoding the mature form of human IL7 (Asp26-His177) was expressed with a N-terminal Met.

Human

The recombinant human IL7 consists of 153 amino acids and predicts a molecular mass of 17.5 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
Immobilized human IL7 at 10 μg/ml (100 μl/well) can bind human IL7Ra-Fch (Cat:503420), The EC₅₀ of human IL7Ra-Fch (Cat:503420) is 15.2-35.6 ng/ml.
2. Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood lymphocytes (PBL). The ED₅₀ for this effect is typically 1.2-4.7 ng/mL.