Human GPHA2 / glycoprotein hormone alpha 2 (His Tag)

Human GPHA2 / glycoprotein hormone alpha 2 (His Tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

GPHA2 is a member of the glycoprotein hormones subunit alpha family. Glycoprotein hormones consist of two subunits, the common alpha- and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in human: FSH, LH, TSH, and CG. GPHA2 and glycoprotein hormone beta 5 (GPHB5) can form a noncovalent heterodimer. GPHA2 can be detected in a variety of tissues. Recombinant A2/B5 heterodimeric glycoproteins activates human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named as thyrostimulin based on its thyroid-stimulating activity. The expression of thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism.

Glycoprotein hormone alpha-2

GLYCOPROTEIN HORMONE ALPHA 2; A2; CYSTEINE KNOT PROTEIN; THYROSTIMULIN SUBUNIT ALPHA; ZSIG51; GLYCOPROTEIN ALPHA 2; GPHA2; PUTATIVE SECRETED PROTEIN ZSIG51; GPA2

Q96T91

GPHA2; GPA2; ZSIG51

Human Cells

A DNA sequence encoding the human GPHA2 (Q96T91) (Met1-Tyr129) was expressed with a polyhistidine tag at the C-terminus.

Human

The recombinant human GPHA2 consists of 117 amino acids and predicts a molecular mass of 13.1 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE