Human Defensin-5 (1-9) peptide

Human Defensin-5 (1-9) peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-ATCYCRTGR-NH2

H-Ala-Thr-Cys-Tyr-Cys-Arg-Thr-Gly-Arg-NH2

9

95.2%

C₄₀H₆₈N₁₆O₁₂S₂

1029.19

Synthetic

Powder

Human alpha-defensin(HD-5) is a strong activity antimicrobial peptide. HD-5(1–9) treatment affects certain bacterial genera and transiently increases microbial diversity in the small intestine. Mice treated orally with HD-5(1–9) had an increased amount of Akkermansia sp. compared with nontreated mice. Moreover, in contrast to other bacteria, Akkermansia muciniphila was not susceptible to HD-5(1–9) in a turbidity broth assay. MS showed that Human Defensin-5 (1-9) had a M.W.=1029.45 dalton

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Ehmann D, Wendler J, Koeninger L, et al. Paneth cell u03b1-defensins HD-5 and HD-6 display differential degradation into active antimicrobial fragments. Proc Natl Acad Sci U S A. 2019;116(9):3746-3751

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.