Human DCAMKL1/DCLK1 recombinant protein

Human DCAMKL1/DCLK1 recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

DCAMKL1, also known as DCLK1, is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.

MKIAA0369; DOUBLECORTIN DOMAIN-CONTAINING PROTEIN 3A; CPG16; CLICK1; CLICK-I; DCLK; DOUBLECORTIN-LIKE AND CAM KINASE-LIKE 1; DOUBLECORTIN AND CAM KINASE-LIKE 1; AI836758; DCL; DCDC3A; RP11-113P14.1; 2810480F11RIK; DCAMKL1; CL1; DOUBLECORTIN AND CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE-LIKE 1; 1700113D08RIK; CLICK-I BETA; SERINE/THREONINE-PROTEIN KINASE DCLK1

Baculovirus-Insect Cells

A DNA sequence encoding the human DCLK1 ( O15075-1) (Met 1-Val 705) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.

Human

The secreted recombinant human DCLK1 consists of 707 amino acids and predicts a molecular mass of 78.5 KDa. The apparent molecular mass of the protein is approximately 64 KDa in SDS-PAGE under reducing conditions due to glycosylation.

> 85 % as determined by SDS-PAGE

Kinase activity untested