Human Betacellulin / BTC recombinant protein

Human Betacellulin / BTC recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Betacellulin(BTC) is a member of the epidermal growth factor (EGF) family. These soluble proteins are ligands for one or more of the four receptor tyrosine kinases encoded by the ErbB gene family (ErbB-1/epidermal growth factor receptor (EGFR), neu/ErbB-2/HER2, ErbB-3/HER3 and ErbB-4/HER4). Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. This protein is a ligand for the EGF receptor. BTC is a polymer of about 62-111 amino acid residues. Secondary Structure: 6% helical (1 helices; 3 residues)36% beta sheet (5 strands; 18 residues). BTC was originally identified as a growth-promoting factor in mouse pancreatic β-cell carcinoma cell line and has since been identified in humans. It plays a role in the growth and development of the neonate and/or mammary gland function. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Probetacellulin

PROBETACELLULIN; BCN; BTC; BETACELLULIN

P35070

BTC

Human Cells

A DNA sequence encoding the human BTC (P35070) (Met1-Tyr111) was expressed with six amino acids (LEVLFQ) at the C-terminus.

Human

The recombinant human BTC comprises 87 amino acids and has a predicted molecular mass of 9.8 kDa. The apparent molecular mass of the protein is approximately 24 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.
The ED₅₀ for this effect is typically 0.2-1 ng/mL.