Hs-1 peptide

Hs-1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

FLPLILPSIVTALSSFLKQG

Phe-Leu-Pro-Leu-Ile-Leu-Pro-Ser-Ile-Val-Thr-Ala-Leu-Ser-Ser-Phe-Leu-Lys-Gln-Gly

20

95.9%

C₁₀₄H₁₇₀N₂₂O₂₆

2144.59

1799386-27-8

Synthetic

Powder

The first AMP from Hypsiboas semilineatus. The sequence of Hs-1 is 55% similar to insect Crabrolin. Active against Gram+ bacteria S. aureus ATCC 33591 (MRSA), B.cereus ATCC 14579, B. subtilis ATCC 23858, L. monocytogenes ATCC 7644 (MIC 12-47 uM). Hemolytic HL50=82 uM. Predicted to be helical. Hs-1 was found in skin, Hypsiboas semilineatus, Brazil, South America. Hs-1 is an antimicrobial peptide displays 80% protection against the dengue-2 virus

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Férir G, Petrova MI, Andrei G, et al. The lantibiotic peptide labyrinthopeptin A1 demonstrates broad anti-HIV and anti-HSV activity with potential for microbicidal applications. PLoS ONE. 2013;8(5):e64010.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.