Hp1404 peptide

Hp1404 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

GILGKLWEGVKSIF

Gly-Ile-Leu-Gly-Lys-Leu-Trp-Glu-Gly-Val-Lys-Ser-Ile-Phe

14

96.8%

Synthetic

Powder

~82% similar to AP2334: scorpion Hp1036. Active against only tested Gram-positive bacteria, including S. aureus AB94004 (also others strains ATCC25923, ATCC6538, AB208193), S. epidermidis AB208187 (also AB208188), M. luteus AB93113, B. subtilis AB91021, S. aureus MRSA P1381, S. aureus MRSA P1374, E. faecium, and S. agalactiae (MIC 4-16 uM). Animal model:mouse peritonitis model, the injected peptide did protect the mice in vivo compared to the untreated group. Serum, but not salt, might have reduced peptide activity in vivo (Salt-insensitive but serum-sensitive). Hp1404 was found in Heterometrus petersii.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Li Z, Xu X, Meng L, et al. Hp1404, a new antimicrobial peptide from the scorpion Heterometrus petersii. PLoS ONE. 2014;9(5):e97539.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.