HOXB7 8-25 peptide

HOXB7 8-25 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-PLLLKLLKSVGAQKD-OH

H-Pro-Leu-Leu-Leu-Lys-Leu-Leu-Lys-Ser-Val-Gly-Ala-Gln-Lys-Asp-OH

15

95.91%

C₇₅H₁₃₅N₁₉O₂₀

1622.98

Synthetic

Powder

HOXB7 8–25 is a novel Homeobox B7 (HOXB7) derived peptide epitope, identified as having the potential to bind to multiple HLA-DRs (DR1, 4, 7, 11, and 15) and target HOXB7 expressing tumours through immune cells. HOXB7 is a master regulatory gene that regulates cell proliferation and activates oncogenic pathways, and overexpression of HOXB7 correlates with poor prognosis in cancer. In human head and neck squamous cell carcinoma (HNSCC) tissue HOXB7 8-25 shows direct cytotoxicity and antigen specific and tumour reactive promiscuous CD4+ T cell responses, producing γ-interferon and directly killing tumours through granzyme B. HOXB7 is thus demonstrated to be a potential target for cancer immunotherapy,

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Komatsuda et al (2022) Mitogen-activated protein kinase inhibition augments the T cell response against HOXB7-expressing tumor through human leukocyte antigen upregulation. Cancer Sci. 2022 Epub ahead of print. PMID: 36285482

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.