Recombinant HLAE protein (His tag)

• DataSheet
• COA (Batch NP456482)
• Material Safety Data Sheets (MSDS)

Recombinant HLA-E Fragment (aa 22-305) – a high-purity, functional fragment of the human HLA class I histocompatibility antigen, alpha chain E, encompassing the Ig-like C1-type domain. This product is ideal for investigating immune regulation, peptide presentation, and HLA-E-mediated signaling in natural killer (NK) and T cell responses.

HLA-E is a non-classical major histocompatibility class Ib molecule critical for immune self-nonself discrimination. In complex with beta-2-microglobulin, it binds self-peptides derived from classical MHC class I signal sequences, serving as a ligand for NK cell inhibitory receptors like KLRD1-KLRC1 to maintain self-tolerance. Under cellular stress or infection, HLA-E presents pathogen-derived peptides (e.g., from HIV, mycobacteria, or SARS-CoV-2) to unconventional CD8-positive T cells, triggering antimicrobial responses. It also plays roles in viral immune evasion (e.g., by human cytomegalovirus and HIV-1) and maternal-fetal tolerance. The full-length protein is primarily localized to the cell membrane as a single-pass type I protein, with a soluble form (sHLA-E) secreted into the extracellular space. The recombinant fragment (aa 22-305) includes the functional Ig-like C1-type domain, enabling focused studies on peptide binding and receptor interactions.

MSKIKGSHSL KYFHTSVSRP GRGEPRFISV GYVDDTQFVR FDNDAASPRM VPRAPWMEQE GSEYWDRETR SARDTAQIFR
VNLRTLRGYY NQSEAGSHTL QWMHGCELGP DGRFLRGYEQ FAYDGKDYLT LNEDLRSWTA VDTAAQISEQ KSNDASEAEH
QRAYLEDTCV EWLHKYLEKG KETLLHLEPP KTHVTHHPIS DHEATLRCWA LGFYPAEITL TWQQDGEGHT QDTELVETRP
AGDGTFQKWA AVVVPSGEEQ RYTCHVQHEG LPEPVTLRWK PASQPTIPIL EHHHHHH