Histatin 3 peptide

Histatin 3 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-DSHAKRHHGYKRKFHEKHHSHRGYRSNYLYDN-OH

H-Asp-Ser-His-Ala-Lys-Arg-His-His-Gly-Tyr-Lys-Arg-Lys-Phe-His-Glu-Lys-His-His-Ser-His-Arg-Gly-Tyr-Arg-Ser-Asn-Tyr-Leu-Tyr-Asp-Asn-OH

32

95.68%

C₁₇₈H₂₅₈N₆₄O₄₈

4062.33

Synthetic

Powder

Histatin 3 is a 32 amino acid histidine-rich peptide found in human saliva. Histatins function as antimicrobial peptides and are important components of the innate immune system. Histatin 3 has broad spectrum antimicrobial action including activity against gram positive and gram negative bacteria, fungi including Candida albicans and against the human protozoan parasite Leishania. Histatin 3 is post-translationally cleaved to form Histatin 5 and Histatin 8

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Fitzgerald et al (2003) Binding, internalisation and degradation of histatin 3 in histatin-resistant derivatives of Candida albicans. FEMS Microbiol. Lett. 220(2) 247 PMID: 12670688
• Oudhoff et al (2008) Histatins are the major wound-closure stimulating factors in human saliva as identified in a cell culture assay. FASEB J. 22(11) 3805 PMID: 18650243
• Hemadi et al (2017) Salivary proteins and microbiota as biomarkers for early childhood caries risk assessment. Int. J. Oral Sci.9, e1 PMID: 29125139

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.