HFRW peptide
Not For Human Use, Lab Use Only.
Cat.#: 319525
Special Price 0.0 USD
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Product Name
HFRW peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
Palmitoyl-HFRW-NH2
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Sequence
Palmitoyl-His-Phe-Arg-Trp-NH2
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Length (aa)
4
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Peptide Purity (HPLC)
95.3%
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Molecular Formula
C48H71N11O5
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Molecular Weight
882.15
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Source
Synthetic
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Form
Powder
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Description
HFRW is a conserved receptor-binding motif found in the a-MSH unit of the adrenocorticotropic hormone (ACTH). It is important for the activation of the MC2R receptor, which is involved in signaling. The HFRW motif, along with the KKRRP sequence at the a-MSHCLIP interface, is required for the activation of MC2R. Processing of ACTH by PC2 to a-MSH specifically abolishes MC2R signaling. The CLIP sequence of ACTH also has a stabilizing effect on plasma stability, although it is weaker compared to the C-terminal orphan poly-Ser sequence of c3-MSH. The C-terminal sequences of both ACTH and c3-MSH contribute to plasma stability, with the poly-Ser sequence of c3-MSH significantly prolonging plasma half-life and reducing degradation.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
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References
- Löw K, Roulin A, Kunz S. A proopiomelanocortin-derived peptide sequence enhances plasma stability of peptide drugs. FEBS Lett. 2020 Sep;594(17):2840-2866. doi: 10.1002/1873-3468.13855. Epub 2020 Jun 27. PMID: 32506501.
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"