Human Hexokinase 3 (His & GST Tag) recombinant protein

Human Hexokinase 3 (His & GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Hexokinase-3, also known as Hexokinase type III, HKIII and HK3, is a protein which belongs to the hexokinase family. Hexokinase-3 / HK3 is an enzyme which in humans is encoded by the HK2 gene. Hexokinases phosphorylate glucose to produce glucose 6-phosphate, committing glucose to the glycolytic pathway. In mammalian tissues hexokinase exists as four isoenzymes encoded by distinct genes. These proteins are homologous and are organized in two homologous domains, with the exception of hexokinase type IV which has only one. This organization is believed to be the result of a duplication and tandem fusion event involving the gene encoding for the ancestral hexokinase. The gene encodes hexokinase-3. Similar to hexokinases-1 and hexokinases-2, this allosteric enzyme is inhibited by its product glucose 6-phosphate.

Hexokinase-3

HK III; HXK3; HKIII; HK3; HK-III; HEXOKINASE 3; MGC143940

P52790

HK3

Baculovirus-Insect Cells

A DNA sequence encoding the human HK3 (P52790) (Met 1-Val 923) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human HK3/GST chimera consists of 1160 amino acids and has a calculated molecular mass of 127 kDa. It migrates as an approximately 115 kDa band in SDS-PAGE under reducing conditions.

> 93 % as determined by SDS-PAGE

Kinase activity untested