H1N1 HA (His Tag) recombinant protein

H1N1 HA (His Tag) recombinant protein

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Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Hemagglutinin

HRAS1; AI451697; NMF4; IHJ; P21RAS; RASH1; AF093576; C-BAS/HAS; SPNA-1; HAMSV; H-RASIDX; SPH; C-H-RAS; C-HA-RAS1; SPNA1; CTLO; HA

P03453

HA

Baculovirus-Insect Cells

A DNA sequence encoding the Influenza A virus (A/USSR/90/77(H1N1)) hemagglutinin (P03453.2) (Met1-Gln529) was expressed with a C-terminal polyhistidine tag.

H1N1

The recombinant hemagglutinin of Influenza A virus (A/USSR/90/1977(H1N1))comprises 523 amino acids and has a predicted molecular mass of 59.4 kDa. The apparent molecular mass of the protein is approximately 65.3 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.01-0.04 μg/mL for 1% GRBC.