H5N1 HA (His & Fc Tag) recombinant protein

H5N1 HA (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).

Hemagglutinin

H-RASIDX; HAMSV; SPNA-1; C-BAS/HAS; CTLO; HA; C-HA-RAS1; SPH; C-H-RAS; SPNA1; AI451697; IHJ; NMF4; P21RAS; HRAS1; RASH1; AF093576

Q5EP31

HA

Human Cells

A DNA sequence encoding the extracellular domain of hemagglutinin (Influenza A virus (A/Viet Nam/1203/2004 (H5N1)) (AAW80717.1) (Met 1-Gln 531) with cleavage site mutated (RERRRKKR?TETR,HA1+HA2,uncleaved) was fused with the C-terminal polyhistidine-tagged Fc region of mouse IgG1 at the C-terminus.

H5N1

Recombinant HA/mFc is a disulfide-linked homodimeric protein. The reduced monomer consisting of 754 amino acids has a calculated molecular mass of 85.7 kDa. As a result of glycosylation, the HA/mFc monomer migrates as an approximately 110-120 kDa protein in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE