H1N1 HA (His Tag) recombinant protein

H1N1 HA (His Tag) recombinant protein

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Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Hemagglutinin

RASH1; AF093576; HRAS1; AI451697; NMF4; IHJ; P21RAS; C-H-RAS; SPH; C-HA-RAS1; SPNA1; CTLO; HA; SPNA-1; C-BAS/HAS; H-RASIDX; HAMSV

C3W627

HA

Human Cells

A DNA sequence encoding the extracellular domain of Influenza A virus H1N1 (A/California/07/2009) (ACP44189.1) hemagglutinin (Met 1-Gln 529) (HA1+HA2, two amino acids deletion, uncleaved) was fused with a polyhistidine tag at the C-terminus.

H1N1

The secreted recombinant hemagglutinin of Influenza A virus (A/California/07/2009(H1N1)) comprises 521 amino acids with the predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 75-85 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE