H5N1 HA (28 Ser/Trp, His Tag) recombinant protein

H5N1 HA (28 Ser/Trp, His Tag) recombinant protein

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Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).

Hemagglutinin

NMF4; AI451697; IHJ; P21RAS; HRAS1; RASH1; AF093576; HAMSV; H-RASIDX; C-BAS/HAS; SPNA-1; CTLO; HA; C-HA-RAS1; SPH; C-H-RAS; SPNA1

Q4H2E2

HA

Human Cells

A DNA sequence encoding the N-terminal segment (Met 1-Glu 340) of the influenza A H5N1 hemagglutinin (A/Hong kong/213/2003 (H5N1)) (ABP51975.1), termed as HA1, was fused with a C-terminal polyhistidine tag, with one amino acid mutated (28 Ser / Trp).

H5N1

The secreted recombinant HA1 subunit of influenza A H5N1 HA (A/Hong kong/213/03 (H5N1)) comprises 335 amino acids and has a predicted molecular mass of 38 kDa. As a result of glycosylation, it migrates as an approximately 45-50 kDa band in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE