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Product Name
H5N1 HA (His Tag) recombinant protein
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Protein name
Hemagglutinin
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Protein short names
RASH1; AF093576; AI451697; IHJ; NMF4; P21RAS; HRAS1; CTLO; HA; C-HA-RAS1; SPH; C-H-RAS; SPNA1; HAMSV; H-RASIDX; C-BAS/HAS; SPNA-1
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Uniprot ID
L7S370
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Gene Name
HA
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) hemagglutinin (AGC13463.1) (Met1-Gln531) with cleavage site mutated (RRRRK ?TET, HA1+HA2, uncleaved) was expressed with a C-terminal polyhistidine tag.
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Protein Species
H5N1
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Molecular weight
The recombinant hemagglutinin of Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) comprises 524 amino acids and has a predicted molecular mass of 59.8 kDa. The apparent molecular mass of the protein is approximately 58.3 kDa in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 2-8 ng/mL for 1% GRBC. -
Validations
Influenza A H5N1 (A/barnswallow/HongKong/D10-1161/2010) Hemagglutinin Protein (HA1 Subunit) (His Tag) SDS-PAGE
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