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Product Name
H5N1 HA (His Tag) recombinant protein
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Protein name
Hemagglutinin
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Protein short names
RASH1; AF093576; IHJ; AI451697; NMF4; P21RAS; HRAS1; CTLO; HA; C-HA-RAS1; C-H-RAS; SPH; SPNA1; HAMSV; H-RASIDX; C-BAS/HAS; SPNA-1
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Uniprot ID
B0L435
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Gln 531) of the influenza A hemagglutinin (A/chicken/India/NIV33487/06 (H5N1)) (ABQ45850.1) (HA1+HA2, uncleaved, cleavage site mutated (RRRKKR-TETR)) was expressed, fused with a C-terminal polyhistidine tag.
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Protein Species
H5N1
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Molecular weight
The secreted recombinant influenza A H5N1 HA (A/chicken/India/NIV33487/2006 (H5N1)) comprises 524 amino acids and has a predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 94 % as determined by SDS-PAGE
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Activity
Measured by its ability to agglutinate human red blood cells.
HA titer is 10-50 μg/mL for 1% HRBC. -
Validations
Influenza A H5N1 (A/chicken/India/NIV33487/2006) Hemagglutinin / HA Protein (His Tag) SDS-PAGE
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