H3N2 HA recombinant protein (C-His)

H3N2 HA recombinant protein (C-His)

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The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

Hemagglutinin, HA1

BAA08716.1

Human Cells

A DNA sequence encoding the Influenza A virus (A/Guizhou/54/1989)(H3N2)) hemagglutinin (BAA08716.1) (Met1-Arg345), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.

H3N2

The recombinant HA1 subunit of the Influenza A virus (A/Guizhou/54/1989)(H3N2) hemagglutinin consists of 340 amino acids and predicts a molecular mass of 38 kDa.

> 95 % as determined by SDS-PAGE.