Laminin Penta Peptide, H-Tyr-Ile-Gly-Ser-Arg-OH

Laminin Penta Peptide, H-Tyr-Ile-Gly-Ser-Arg-OH

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

YIGSR

H-Tyr-Ile-Gly-Ser-Arg-OH

5

98.62%

C₂₆H₄₂N₈O₈

594.67

Synthetic

Powder

This pentapeptide sequence, located within domain III of the laminin B1 chain, represents a major receptor binding site in laminin. By competing with laminin for the cell surface receptor, the peptide is able to inhibit cell attachment to basement membranes, thus preventing the formation of tumor metastases. The peptide also promotes tumor cell migration and, moreover, mediates the adhesion of a variety of epithelial cells to laminin.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• J.Graf et al., Biochemistry, 26, 6896 (1987)
• Y.Iwamoto et al., Science, 238, 1132 (1987)
• Y.Iwamoto et al., J. Cell. Physiol., 134, 287 (1988)
• G.Hunt, Expl. Cell Biol., 57, 165 (1989)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.