[D-Ala2] Met-Enkephalin, amide peptide

[D-Ala2] Met-Enkephalin, amide peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

YaGFM, a=D-Ala

H-Tyr-D-Ala-Gly-Phe-Met-NH2

5

97.4%

C₂₈H₃₈N₆O₆S

586.71

61370-87-4

Synthetic

Powder

[D-Ala²] Met-Enkephalin, amide is a synthetic peptide analog of the endogenous opioid peptide Met-enkephalin. This modification involves the substitution of the second amino acid residue with a D-alanine, which enhances its stability against enzymatic degradation. The peptide exhibits opioid activity, primarily through interaction with opioid receptors. In experimental models, [D-Ala²] Met-Enkephalin, amide has been shown to inhibit the release of oxytocin. This inhibitory effect is reversible by naloxone, indicating a receptor-mediated mechanism. The peptide's action is observed in response to both cholinergic stimulation and physiological stimuli, suggesting a role in modulating neuroendocrine pathways.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Haldar J, Hoffman DL, Zimmerman EA. Morphine, beta-endorphin and [D-Ala2] Met-enkephalin inhibit oxytocin release by acetylcholine and suckling. Peptides. 1982 Jul-Aug;3(4):663-8. doi: 10.1016/0196-9781(82)90168-1. PMID: 6291011.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.