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Product Name
Mouse PLA2G12B (His Tag) recombinant protein
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Description
Mouse Group XIIB secretory phospholipase A2-like protein, also known as Group XIII secretory phospholipase A2-like protein, GXIII sPLA2-like, sPLA2-GXIIB, GXIIB, PLA2G13 and PLA2G12B, is a secreted protein which belongs to the phospholipase A2 family. PLA2G12B / PLA2G13 is strong expression in liver, small intestine and kidney. Mammalian secretory phospholipase A2s ( sPLA2s ) form a family of structurally related enzymes that are involved in a variety of physiological and pathological processes via the release of arachidonic acid from membrane phospholipids or the binding to specific membrane receptors. Phospholipases A2 / PLA2 are enzymes that release fatty acids from the second carbon group of glycerol. This particular phospholipase specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond releasing arachidonic acid and lysophospholipids. Phospholipases A2 / PLA2 are commonly found in mammalian tissues as well as insect and snake venom. Venom from both snakes and insects is largely composed of melittin, which is a stimulant of Phospholipases A2 / PLA2. Due to the increased presence and activity of Phospholipases A2 / PLA2 resulting from a snake or insect bite, arachidonic acid is released from the phospholipid membrane disproportionately. As a result, inflammation and pain occur at the site.
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Protein name
Group XIIB phospholipase A2-like protein
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Protein short names
PLA2G13; FKSG71; HLB218; PLA2G12B; 2010002E04RIK; MGC138151; GXIIB; GXIIISPLA2
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Uniprot ID
Q8VC81
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Gene Name
Pla2g12b; mCG_11300
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse PLA2G12B (NP_076019.2) (Met 1-Leu 195) was expressed, with a C-terminal polyhistidine tag.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse PLA2G12B comprises 187 amino acids and has a calculated molecular mass of 21 kDa. The apparent molecular mass of the recombinant protein is approximately 24 kDa in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Validations
Mouse PLA2G12B / PLA2G13 Protein (His Tag) SDS-PAGE
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