Human G-CSFR/CD114 (Fc Tag) recombinant protein

Human G-CSFR/CD114 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.

Granulocyte colony-stimulating factor receptor

GCSFR; RP23-140F18.1; MGC141472; MGC141473; G-CSF-R; G-CSFR; CSFGR; CD114; CSF3R

Q99062

CSF3R; GCSFR

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Pro 621) of human G-CSF receptor (NP_000751.1) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus.

Human

The mature recombinant human G-CSFR/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 835 amino acids and predicts a molecular mass of 93.3 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rh GCSFR/Fc monomer is approximately 120-130 kDa due to glycosylation.

> 95 % as determined by SDS-PAGE

Measured by its ability to inhibit the GCSF-induced proliferation of NFS-60 mouse myeloid cells. The ED₅₀ for this effect is typically 1-4 ng/ml in the presence of 0.125 ng/ml of recombinant human GCSF.