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GP repeat antibody

Cat.#: 111052

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Product Information

  • Product Name
    GP repeat antibody
  • Documents
  • Description
    GP repeat Rabbit Polyclonal antibody. Positive WB detected in Recombinant protein. Positive IHC detected in human cerebellum tissue.
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    C9RAN antibody; GP repeat antibody; GPGPGPGP antibody; poly GP antibody; poly PG antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide. Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: N/A

  • Validations

    IHC results of GP-repeated antibody (Catalog No:111052, 1:500) with human cerebellum tissue (C9ORF72 expansion carrier) by Prof. Dr. Manuela Neumann. Staining conditions: Ventana automatic staining system using Optiview DAB detection kit; pretreatment boiling for 30min in citrate buffer pH6.

    IHC results of GP-repeated antibody (Catalog No:111052, 1:500) with human cerebellum tissue (C9ORF72 expansion carrier) by Prof. Dr. Manuela Neumann. Staining conditions: Ventana automatic staining system using Optiview DAB detection kit; pretreatment boiling for 30min in citrate buffer pH6.

    Recombinant proteins of SMARCA4 which contains GP repeat sequence were subjected to SDS PAGE followed by western blot with Catalog No:111052 (GP repeat Antibody) at dilution of 1:1000

    Recombinant proteins of SMARCA4 which contains GP repeat sequence were subjected to SDS PAGE followed by western blot with Catalog No:111052 (GP repeat Antibody) at dilution of 1:1000

  • Background
    The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". This antibody is used to detect the GP repeated sequence. The recombinant protein 6*His tag-SMARCA4 which contains the sequence “GPGPGPGPG” was used to detect this antibody.
  • References
    • Cooper-Knock J, Higginbottom A, Stopford MJ. Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy. Acta neuropathologica. 130(1):63-75. 2015.
    • Mackenzie IR, Frick P, Grässer FA. Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers. Acta neuropathologica. 130(6):845-61. 2015.
    • Davidson Y, Robinson AC, Liu X. Neurodegeneration in frontotemporal lobar degeneration and motor neurone disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins. Neuropathology and applied neurobiology. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"