(Glu1)-Fibrinopeptide B

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Human fibrinopeptide B (hFpB) is a 14-amino acid peptide cleaved from the amino-terminus of the fibrinogen Bβ-chain by the action of thrombin. It is a proteolytic product generated during fibrin formation and is also contained within the primary plasmin cleavage fragment of fibrinogen, Bβ 1-42. This peptide exhibits potent chemotactic activity for human neutrophils (PMN), with optimal activity observed at approximately 10^-8 M. Its chemotactic potency for neutrophils is comparable to that of well-established chemoattractants such as C5a, leukotriene B₄, and formyl-methionyl-leucyl-phenylalanine.

Studies indicate that hFpB stimulates directed migration of neutrophils and fibroblasts. The chemotactic response to hFpB is specific, as it is blocked by pre-incubation with anti-hFpB antiserum. Furthermore, evidence suggests hFpB acts through a receptor mechanism distinct from those used by C5a, LTB₄, or fMLP. Like fMLP, hFpB induces a rapid, dose-dependent increase in cytoskeletal-associated actin in neutrophils. However, unlike fMLP, hFpB does not trigger neutrophil aggregation, lysosomal enzyme release, or superoxide anion production. This profile indicates hFpB can recruit specific cell types without concurrently activating certain pro-inflammatory secretory pathways.

• Senior RM, Skogen WF, Griffin GL, Wilner GD. Effects of fibrinogen derivatives upon the inflammatory response. Studies with human fibrinopeptide B. J Clin Invest. 1986 Mar;77(3):1014-9. doi: 10.1172/JCI112353. PMID: 3005361; PMCID: PMC423507.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.