-
Product Name
EV71 EV71 VP0 (His & GST Tag) recombinant protein
- Documents
-
Description
Capsid protein VP1: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor to provide virion attachment to target host cells. This attachment induces virion internalization. Tyrosine kinases are probably involved in the entry process. After binding to its receptor, the capsid undergoes conformational changes. Capsid protein VP1 N-terminus (that contains an amphipathic alpha-helix) and capsid protein VP4 are externalized. Together, they shape a pore in the host membrane through which viral genome is translocated to host cell cytoplasm. After genome has been released, the channel shrinks (By similarity).
-
Protein name
Genome polyprotein
-
Protein short names
P2A; P2B; P2C; P3A; VPg; P3B; P3C; RdRp; 3Dpol; 3D
-
Uniprot ID
Q66478
-
Source/Expression Host
Baculovirus-Insect Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the amino acids (Met 1-Gln 323) of human enterovirus 71 genome polyprotein (Q66478-1), corresponding to the protein VP0 precusor, a component of immature procapsids, was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
-
Protein Species
EV71
-
Molecular weight
The recombinant human enterovirus 71 protein VP0/GST chimera consists of 560 amino acids and has a calculated molecular mass of 63 kDa. It migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions.
-
Purity
> 72 % as determined by SDS-PAGE
-
Validations
Human Enterovirus 71 VP0 Protein (His & GST Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"