Human B3GAT3 / GLCATI (His Tag) recombinant protein

Human B3GAT3 / GLCATI (His Tag) recombinant protein

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Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation.

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3

SQV-8-LIKE PROTEIN; UDP-GLCUA:GAL BETA-1; GLCAT-I; GLCUAT-I; 3-GLUCURONYLTRANSFERASE 3 (GLUCURONOSYLTRANSFERASE I); GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE 3; 2810405M13RIK; 3-GAL-R GLUCURONYLTRANSFERASE; GLUCURONOSYLTRANSFERASE I; GLCATI; GLUCURONYLTRANSFERASE I; BETA-1; B3GAT3

O94766

B3GAT3

E. coli

A DNA sequence encoding the human B3GAT3 (NP_036332.2) (Glu72-Val335) was expressed with a polyhistidine tag at the N-terminus.

Human

The recombinant human B3GAT3 consists of 282 amino acids and predicts a molecular mass of 31.5 KDa. It migrates as an approximately 33 KDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE